Intended Use
Cucumber mosaic virus (CMV) causes serious diseases of many economically important plants, particularly cucumber
and other cucurbits (squashes and pumpkins). The virus can also cause damage to many herbaceous and woody
ornamentals such as Begonia, Pelargonium, Gladiolus, Potato vine, lilies, delphiniums, primulas and passion flower.
There are no chemicals that will cure a plant of CMV.
A wide range of plant material can be tested such as leaves, flowers and the skin of suspected fruit.
Cucumber mosaic virus Pocket Diagnostic is a rapid diagnostic test designed to be used in the field, nursery or
glasshouse. The test can be performed within minutes by operators with little or no training. Samples for testing can
be taken from any part of the plant, at any stage during the production cycle. Pocket Diagnostic test results form an
important part of sound decision making in routine screening or disease control situations.

Cucumber mosaic virus Test Kit Contents:
· Test device (x4)
· Bottle of extraction buffer (including ball bearing) (x4)
· Pipette (x4)
· Instructions for Use
Quality Standards
Pocket Diagnostic tests are manufactured in environmentally-controlled facilities by Forsite Diagnostics Ltd, trading
as Abingdon Health, following procedures which meet ISO9001 quality standards and where possible validated to the
European and Mediterranean Plant Protection Organisation (EPPO) standards.
Storage and Shelf life
Pocket Diagnostic Kits should be stored at room temperature (do not exceed 40°C). Tests are supplied with at least 6
months shelf life.

Safety
The Pocket Diagnostic test device contains no hazardous materials to humans, animals, plants or crops. The
accompanying extraction buffer contains a very small amount of sodium azide (0.05%) as a preservative – avoid
ingestion and skin contact. Refer to your government guidelines for the disposal of sodium azide.
Disposal of used tests
In cases where positive or suspect positive results are obtained for quarantine or notifiable pathogens, all kit
components should be regarded as contaminated and should be disposed of appropriately. Contact the Food and
Environment Research Agency (FERA) for further details on how to do this. If you are not based in the UK please
contact your government plant pathology and plant pathogen quarantine department.
Test Procedure
1. Select sample. Where possible select a part of the plant where diseased and healthy material meet, do not use
dead material. Use approximately 25mm square of material (See picture 1). Take care to avoid cross
contamination between samples from hands or cutting tools.
2. Cut or tear sample into small pieces and put into bottle.
3. Shake firmly for 30 seconds (longer for more resistant samples)

4. Draw liquid into pipette.
5. Add 2-3 drops into sample well of test strip.
6. Read result after 5-10 minutes.

Interpreting results
A line present next to the T (test line) indicates a positive result.
If the C line (control) does not appear the test has failed and must be repeated.
Ignore any changes to the result which occur after the 10 minutes has elapsed.