Equipment
What is PrIEF3000?
PrIEF is a product based on Free Flow Electrophoresis (FFE) technology, and can be used for fractionation and enrichment of any charged or chargeable molecules/particles, such as :u Cell uCell organelles uProteins and Protein Isoforms uPeptides u Membrane proteins
Modes of Separation
PrIEF3000 can be used for three modes of operation which are used for different application.These three modes of the separation is based on different physicochemical properties (please refer to the following figures and table):
Modes of operation IEF (isoelectric focusing) ZE (zone electrophoresis) ITP (isotachophoresis)
Figures 
Separation based on PI (Isoelectric Point) Net charge density Electrophoretic mobility
(charge, size and shape)
pH profile Linear gradient Constant pH Step gradient
Application Mainly for separation of Separation of bio-particles Analysis of traces in samples
proteins and peptides. like cells and organelles like protein isoforms and
membrane protein
Application possibilities (Typical articles referring to FFE methods)
Methods Review
1. Moritz RL, Simpson RJ.(2005) Liquid-based free-flow electrophoresis- reversed-phase HPLC: a proteomic tool. Nat Methods.2:863-73.
Cell organelles separation
2. Zischka, H., Weber, G., Weber P.J.A., Posch, A., Braun, R.J., Bühringer, D., Schneider, U., Nissum, M., Meitinger, D.,Ueffing, M. and C. Eckerskorn. (2003) Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresis. Proteomics, 906-916.
Membrane protein separation
3. Gerbeau, P., Guclu, J., Ripoche, P. and Maurel, C. (1999) Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes, Plant J. 18 577-587.
Protein separation
4. Xie H, Rhodus NL, Griffin RJ, Carlis JV, Griffin TJ.（2005）A catalogue of human saliva proteins identified by free flow electrophoresis-based peptide separation and tandem mass spectrometry. Mol Cell Proteomics. 4:1826-30.
Protein isoforms separation
5. Glukhovski, P. and Vigh, G. (1999) Analytical and preparative scale isoelectric focusing separation of enantiomeres, Anal Chem 713814-3820.
