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Email: service@joyingbio.com

Address:Room 1503-3,

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Xiaoshan Economic And Technology Development Zone,

Xiaoshan District, Hangzhou, Zhejiang



Demeter PrIEF3000 Continuous Free Flow Electrophoresis System

What is PrIEF3000?

PrIEF is a product based on Free Flow Electrophoresis (FFE) technology, and can be used for fractionation and enrichment of any charged or chargeable molecules/particles, such as :u Cell uCell organelles uProteins and Protein Isoforms uPeptides u Membrane proteins

Modes of Separation
PrIEF3000 can be used for three modes of operation which are used for different application.These three modes of the separation is based on different physicochemical properties (please refer to the following figures and table):

Modes of operation         IEF (isoelectric focusing)               ZE (zone electrophoresis)                 ITP (isotachophoresis)


Separation based on       PI (Isoelectric Point)                           Net charge density                          Electrophoretic mobility

                                                                                                             (charge, size and shape)

pH profile                            Linear gradient                                    Constant pH                                          Step gradient

Application                         Mainly for separation of                      Separation of bio-particles            Analysis of traces in samples

                                              proteins and peptides.                       like cells and organelles                like protein isoforms and

                                                                                                                                                               membrane protein

Application possibilities (Typical articles referring to FFE methods)

Methods Review

1. Moritz RL, Simpson RJ.(2005) Liquid-based free-flow electrophoresis- reversed-phase HPLC: a proteomic tool. Nat Methods.2:863-73.

Cell organelles separation

2. Zischka, H., Weber, G., Weber P.J.A., Posch, A., Braun, R.J., Bühringer, D., Schneider, U., Nissum, M., Meitinger, D.,Ueffing, M. and C. Eckerskorn. (2003) Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresis. Proteomics, 906-916.

Membrane protein separation

3. Gerbeau, P., Guclu, J., Ripoche, P. and Maurel, C. (1999) Aquaporin Nt-TIPa can account for the high permeability of tobacco cell vacuolar membrane to small neutral solutes, Plant J. 18 577-587.

Protein separation

4. Xie H, Rhodus NL, Griffin RJ, Carlis JV, Griffin TJ.(2005)A catalogue of human saliva proteins identified by free flow electrophoresis-based peptide separation and tandem mass spectrometry. Mol Cell Proteomics. 4:1826-30.

Protein isoforms separation

5. Glukhovski, P. and Vigh, G. (1999) Analytical and preparative scale isoelectric focusing separation of enantiomeres, Anal Chem 713814-3820.